Integrated analysisof monocyte infiltration and differential gene expressionin hypertrophic obstructive cardiomyopa-thy
QIN Xian-yu1
TAN Jian1
ZHENG Hao-sheng1
ZHENG Yu-zhen1
LIAO Hong-ying1
ZHUANG Jian2
1.Department of Thoracic Surgery,Thoracic Cancer Center,The Sixth Affiliated Hospital,Sun Yat-sen University,Guangzhou 510655,Guangdong,China;Biomedical Innovation Center,The Sixth Affiliated Hospital,Sun Yat-sen University,Guangzhou 510655,China2.Guangdong Cardiovascular Institute,Guangdong Provincial Key Laboratory of South China Structural Heart Dis-ease,Guangdong Provincial People's Hospital,Guangdong Academy of Medical Sciences,Guangzhou 510100,Guangdong Province,China
摘要：Background Hypertrophic obstructive cardiomyopathy(HOCM)is one of the main reasons for sudden cardiac death(SCD)in young people.Researches has revealed that immune-related genes are closely relevant to HOC-Mprogression.Therefore,it is important to explore the key immuneregulatory mechanisms and biomarkersof HOCM progression.Methods The bioinformatics methods,including linear models for microarray analysis(LIMMA),protein-protein interaction(PPI)network,Gene Ontology(GO),Kyoto Encyclopedia of Genes and Ge-nomes pathway(KEGG)and CIBERSORT,were used to assess the key pathways and hub genes involved in HOCM.Furthermore,expression levels of hub genes were validatedin human tissue.Results Our results showed that the degree of infiltration of five immune cells was linked to HOCM progression,including monocytes,macro-phages M2,natural killer(NK)cell resting,B cells native,and T cells regulatory(Tregs).A total of 7 hub genes(CCL2,CXCL8,FOS,MAP2K1,NFKBIA,STAT3,and TNFRSF1A)were identified and validated by quantitative real-time polymerase chain reaction(qt-PCR).The core genes including CCL2,MAP2K1,NFKBIA,STAT3,and TNFRSF1A are closely related to monocytes infiltration during HOCM progression.Conclusions Taken togeth-er,our research provided useful information to explore the immune mechanisms underlying HCM progression and to provide a potential therapeutic target for therapy in HOCM.The interactional relationship of GATA5,BCL3,and ATF1 complex-regulation CCL2,MAP2K1,NFKBIA,STAT3,and TNFRSF1A was involved in the regulation of monocytes tissue infiltration,which was closely related to the progression of HOCM.[S Chin J Cardiol 2024;25(4):260-274]
机标关键词：integratedgeneanalysisofcardiomyopa-thydifferentialexpressioninhypertrophicinfiltration
论文发表日期：2024-12-30
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：15( 260-274 )
英文信息