Liraglutide directly protects cardiomyocytes against reperfusion injury possibly via modulation of intracellular calcium homeostasis
Shun-Ying HU
Ying ZHANG
Ping-Jun ZHU
Hao ZHOU
Yun-Dai CHEN
摘要：Background Limglutide is glucagon-like peptide-1 receptor agonist for treating patients with type 2 diabetes mellitus.Our previous studies have demonstrated that limglutide protects cardiac function through improving endothelial function in patients with acute myocardial infarction undergoing percutaneous coronary intervention.The present study will investigate whether liraglutide can perform direct protective effects on cardiomyocytes against reperfusion injury.Methods In vitro experiments were performed using H9C2 cells and neonatal rat ventricular cadiomyocytes undergoing simulative hypoxia/reoxygenation (H/R) induction.Cardiomyocytes apoptosis was detected by fluorescence TUNEL.Mitochondrial membrane potential (⊿ψm) and intracellular reactive oxygen species (ROS) was assessed by JC-1 and DHE,respectively.Fura-2/AM was used to measure intracellular Ca2+ concentration and calcium transient.Immunofluorescence staining was used to assess the expression level of sarcoplasmic reticulum Ca2+-ATPase (SERCA2a).In vivo experiments,myocardial apoptosis and expression of SERCA2a were detected by colorimetric TUNEL and by immunofluorescence staining,respectively.Results In vitro liraglutide inhibited cardiomyotes apoptosis against H-/R.⊿ψm of cardiomyocytes was higher in liraglutide group than H/R group.H/R increased ROS production in H9C2 cells which was attenuated by liraglutide.Liraglutide significantly lowered Ca2+ overload and improved calcium transient compared with H/R group.Immunofluorescence staining results showed limglutide promoted SERCA2a expression which was decreased in H-/R group.In ischemia/reperfusion rat hearts,apoptosis was significantly attenuated and SERCA2a expression was increased by liraglutide compared with H/R group.Conclusions Liraglutide can directly protect cardiomyocytes against reperfusion injury which is possibly through modulation ofintracellular calcium homeostasis.
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论文发表日期：2017-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：10( 57-66 )
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