Effect of Allocryptopine on Late sodium current of atrial myocytes in spontaneously hypertensive rats
Ying ZHAO
Xiao-Ting XIE
Yan-Mei SUN
Zhong-Qi CAI
Ying DONG
Chao ZHU
Xi CHEN
Hong-Lin WU
Jian-Cheng ZHANG
Yang LI
摘要：Objective To explore the effect of allocryptopine (All) on the Late sodium current (INa,Late) of atrial myocytes in spontaneously hypertensive rats (SHR).Method The enzyme digestion method was used to separate single atrial myocytes from SHR and Wistar-Kyoto rat (WKY) rats.INa,Late was record by patch-clamp technique and the effect of All on the current was evaluated.Results Comparing with WKY cells,markedly increasing of INa,Late current in SHR myocytes was found from 0.24 ± 0.02 pA/pF of WKY cells to 1.73 ± 0.04 pA/pF of SHR cells (P ＜ 0.01,n =15).After treament with 30 μmol/L All;the current densities was reduced to 0.92 ± 0.03 pA/pF.The ratio of INa,Late/INa,peak of WKY and SHR were 0.09％ ± 0.01％ and 0.71％ ± 0.02％,INa,Late/INa,peak of SHR was reduced to 0.37％ ± 0.02％ by 30 μrnol/L All (P ＜ 0.01,n =15).We also determined the effect of All on the gating mechanism of the INa,Late in the SHR cells.It was found that All decreased the INa,Late by alleviating the inactivation of the channels and increasing the window current of sodium channel.Conclusion Increased INa,Late in SHR atrial myocytes and the prolonged APD were inhibited by All coming from Chinese herb medicine.
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论文发表日期：2017-10-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：7( 280-286 )
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