Trehalose attenuates abdominal aortic aneurysm formation by inducing autophagy in smooth muscle cells
Bo JIANG1
Xuan LI1
Mo WANG2
Guang-Xin LI3
Peng-Wei REN2
Yu-Qi WANG4
Shi-Jie XIN1
Ling-Feng QIN2
1.Department of Vascular Surgery,the First Hospital of China Medical University,Key Laboratory of Pathogenesis,Pre-vention and Therapeutics of Aortic Aneurysm,Shenyang,China2.Department of Surgery,Yale School of Medicine,New Haven,CT,USA3.Department of Breast and Thyroid Surgery,Peking University Shenzhen Hospital,Shenzhen,China4.Yale Stem Cell Center,Yale School of Medicine,New Haven,CT,USA;Department of Biomedical Engineer-ing,Yale University,New Haven,CT,USA
摘要：BACKGROUND Trehalose is a naturally occurring disaccharide, which has been identified as an autophagy inducer and ex-hibits protective effect in cardiovascular diseases such as myocardial infraction and atherosclerosis. However, the functional role of trehalose in abdominal aortic aneurysm (AAA) remains undefined. METHODS To study the effect of trehalose in AAA, trehalose (1 g/kg per day) were given for 14 continuous days in a mouse model of elastase-induced abdominal aortic aneurysm. On day 14, ultrasound was performed to measure aortic diameter before the abdominal aortas were harvested and processed for further analysis. Verhoeff-Van Gieson staining and TUNEL staining were performed on paraffin sections to evaluate vascular histology and apoptosis, immunofluorescence staining and Western-blot were performed to evaluate expression of autophagy markers. RESULTS Echocardiography and in situ pictures demonstrated that trehalose attenuated infrarenal aorta dilation. Verhoeff-Van Gieson staining showed elastin degradation was improved in trehalose-treated group. Compared with vehicle-treated mice, trehalose treatment restored smooth muscle cell contractile phenotype with increased α-SMA, Calponin and Myh11 expression. Furthermore, trehalose also attenuated cell apoptosis and leukocytes infiltration. Importantly, trehalose induced autophagy with decrease SQSTM1/p62 accumulation, increased lamp2 expression and LC3B conversion. CONCLUSION Trehalose attenuated AAA progression with decreased inflammation and restored SMC contractile phenotype by inducing autophagy. These results demonstrated the therapeutic potential of trehalose in AAA.
机标关键词：trehalosecellsabdominalaneurysmaorticattenuatesautophagyformation
论文发表日期：2023-03-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：9( 214-222 )