Circulating circRNA expression profile and its potential role in late recurrence of paroxysmal atrial fibrillation post cath-eter ablation
Shan-Shan LIU1
Hong-Yang GUO2
Jian ZHU1
Jin-Ling MA1
Sai-Zhe LIU2
Kun-Lun HE3
Su-Yan BIAN1
1.Department of Cardiology,the Second Medical Center,National Clinical Research Center of Geriatric Disease,Beijing Key Laboratory of Chronic Heart Failure Precision Medicine,Chinese PLA General Hospital,Beijing,China2.Depart-ment of Cardiology,the Six Medical Center,Chinese PLA General Hospital,Beijing,China3.Medical Big Data Re-search Center,Medical Innovation Research Department,Beijing Key Laboratory of Chronic Heart Failure Precision Medi-cine,Chinese PLA General Hospital,Beijing,China
摘要：BACKGROUND Catheter-based pulmonary vein isolation(PVI)is an effective and well-established intervention for sympto-matic paroxysmal atrial fibrillation(PAF).Nevertheless,late recurrences of atrial fibrillation(LRAF)occurring during 3 to 12 months are common,and the underlying mechanisms remain elusive.Circular RNAs(circRNAs)in atrial tissue have been linked to the pathophysiological mechanisms and progression of PAF in a few studies.However,their expression patterns in peripheral blood and regulatory function in LRAF are not clear.
METHODS In the present study,the expression profile of circulating circRNAs in three paired nonvalvular PAF patients with or without LRAF was investigated by high-throughput sequencing and validated by quantitative real-time polymerase chain re-action(qRT-PCR).Bioinformatics analyses,including Gene Ontology(GO),Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis,and circRNA/miRNA regulatory network,were performed to predict the functions and potential regulatory roles of differentially expressed(DE)circRNAs.
RESULTS A total of 12,834 circRNAs,comprising 5,491 down-regulated and 7,343 up-regulated circRNAs,were found to be DE in blood smaples from the two groups in peripheral blood between LRAF and non-recurrence control individuals.The most en-riched GO categories in terms of molecular function,biological process,and cellular component features were catalytic activity,cellular metabolic process,and intracellular part,respectively.The KEGG enrichment study revealed that the most important metabolic process controlled by DE circRNAs is endocytosis.In the circRNA/microRNAs interaction network,four up-regulated circRNAs(hsa_circ_0002665,hsa_circ_0001953,hsa_circ_0003831,and hsa_circ_0040533)and one down-regulated circRNA(hsa_circ_0041103)were predicted to play potential regulatory roles in the pathogenesis of LRAF.
CONCLUSIONS This investigation discovered the expression pattern of circulating circRNAs that is indicative of PAF late re-currence,which may serve as risk markers or therapeutic targets for LRAF after PVI.
机标关键词：expressioncircrnaprofilelatepostroleablationatrial
论文发表日期：2023-11-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：13( 788-800 )