Proliferative potential and angiogenic characteristics of blood outgrowth endothelial cells derived from middle-aged and older adults
Xue-Juan XIA1
Xuan-Yu CHEN2
Lin-Lin XIAO3
1.School of Health Science and Engineering,University of Shanghai for Science and Technology,Shanghai,China;Department of Food Science & Technology,National University of Singapore,Singapore,Singapore2.School of Health Science and Engineering,University of Shanghai for Science and Technology,Shanghai,China3.Department of Laboratory Medicine,Affiliated Sixth People's Hospital South Campus,Shanghai Jiaotong University,Shanghai,China
摘要：Objectives Autologous blood outgrowth endothelial cells(BOECs)have been proposed to induce therapeutic angiogenesis for treating cardiovascular diseases(CVDs).The aim of the present study was to investigate the proliferative potential and angiogen-ic characteristics of BOECs among middle-aged and older adults,the population particularly susceptible to CVDs.
Methods BOECs were isolated from 48 peripheral blood samples of subjects aged 56±4 years.The cells were then distin-guished based on their proliferative abilities,and their phenotype,tube formation capacity,and migratory activity were com-pared using immunofluorescence staining,flow cytometry,tube formation assay,and wound healing assay,respectively.Correl-ations between demographic,clinical,and dietary parameters with the number of BOECs were also assessed.
Results A total of 132 BOEC colonies with different proliferative potentials were obtained,including colonies lost proliferative ability before passage 3(named LPA),stopped proliferating during passage 3-8(HPA(3-8)),and proliferated after passage 8(HPA(＞8)).LPA cells appeared later and displayed abnormal morphology,while HPA(3-8)cells exhibited alterations in von Willebrand factor morphology and lower KDR expression.HPA(＞8)cells obtained higher branching intervals and individual cell migration velocity compared with those of HPA(3-8)cells.Correlation analysis showed that the number of both LPA and HPA colonies were positively associated with several CVD risk factors.Additionally,the number of LPA colonies was positively associated with servings of meats and alternatives,fruits,fruits and vegetables,as well as the protein intake.
Conclusions Our findings provide evidence that the middle-aged and older populations possess BOECs with different prolifer-ative and angiogenic potentials,exhibiting distinctions in cell morphology,appearance dates,VWF morphology,and KDR ex-pression.Strikingly,a higher number of BOECs is likely associated with an increased risk of CVDs,while the number of BOECs with low proliferative ability may be regulated by diet.
机标关键词：characteristicscellsbloodfromadultsangiogenicderivedendothelial
论文发表日期：2024-11-28
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：14( 1071-1084 )