The Collapsing Activity of a Neuron Growth Inhibitor Collapsin-1 Could be Neutralized by Its Antibody
Du Ailian
Ding Xinsheng
Deng Xiaoxuan
Yao Juan
Cheng Hong
摘要：Objective To produce the antiserum of chicken-brain-derived neuron growth in hibitor collapsin-1. To detect its ability to neutralize the collapsing activity of collapsin-1. Methods The c-myc epitope tagged entire sequence of collapsin-1 was amplified by PCR and expression cloned. Rabbits were immunized with the c-myc-tagged collapsin-1 to produce antiserum of collapsin-1. Its ability to neutralize the collapsing activity of collapsin-1 was observed in dorsal root ganglia ( DRG ) growth cone. Results Collapsin-1 could induce the collapse of DRG growth cone. The collapsing activity of collapsin-1 could be neutralized by the antisemm of collapsin-1. Conclusion We produced the antibody of a neuron growth inhibitor-collapsin-1 that could block its inhibiting function.
机标关键词：
分类号：R9(药学)
资助基金：国家自然科学基金(39840011)
论文发表日期：2000-07-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：3( 74-76 )
英文信息