Phenotypical Reversion and Differentiation of Breast Cancer in vitro by Sodium Phenylacetate
Ji Jiaxiang
Lu Zhongmou
Ji Xiaohui
Ma Baoli
摘要：Objective To evaluate the effect of a nontoxic differentiation inducer, sodium phenylacetate (NaPA) on breast cancer MCF-7 and MDA-453 cells in vitro. Methods The anchorage growth assay, ELISA, immunofluorescence analysis and electron microscopy were used to study the effect of NaPA on MCF-7 and MDA-453 cells. Results NaPA treatment resulted in a dose-dependent inhibition of MCF-7 or MDA-453 cell growth, including anchorage-dependent and anchorage-independent growth, but il did not significantly inhibit the growth of normal amnion tissue Wish cells and adult hepatic cells L-02. In addition, NaPA could enhance the expressions of intercellular adhesion molecule-1(ICAM-1), human leukocyte antigen ( HLA ) class Ⅰ and Ⅱ molecules on the surface of MCF-7 and MDA-453 cells. Electron micrographs demonstrated richer rough endoplasmic reticulum,mitochondria in NaPA-treated MCF-7 and MDA-453 cells, in contrast to the scant before-mentwned structures but scattered cytoplasmic polyribosomes in the untreated ones. Conclusion NaPA is effective in inducing phenotype reversion and differentiation of breast cancer, and potential in cancer intervention.
机标关键词：
分类号：R73(肿瘤学)
资助基金：上海市学院博士建设计划(03509000)
论文发表日期：2000-07-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：7( 77-82,90 )
英文信息