Construction of Retroviral Vectors Containing Rat Fas Ligand Gene and FasL Expression Mediated by the Vectors in Hepatocellular Carcinoma Cells
SUN Bei-cheng
WANG Xue-hao
QIAN Jian-min
ZHANG Feng
XU Zhi-xiang
LI Xiang-Cheng
ZHANG Xue-guang
摘要：Objective In order to study the biological function of Fas and Fas ligand system, discuss the feasibility of treating tumor with transfecting FasL gene. Methods the rat Fas ligand complementary DNA was subcloned to retroviral vector pLXSN, acquired pLXSN/FasL+ recombinant with direct inserting and single copy,then packaged with PA317 amphotropic packaging cells,anti-G418 clones were acquired,and it was named PA317/ pLXSN-FasL+ cells.Results The titer of virus was 4.7×107 CFU/ml,there was FasL gene integration in PA317/pLXSN-FasL+ cells detected by polymerase chain reaction. When we used the supernatant of the PA317/pLXSN-FasL+ cells to infect hepatocellular carcinoma cells HepG-2,SMMC-7721,CBRH-7919 and RH-35 ,the FasL expression was found at all the surface of the four cell lines through FCM,and the apoptosis in HepG-2 and CBRH-7919 cells which had high levels Fas expression was found too.Conclusion the results show that it is an effective way to introduce FasL gene to retroviral vectors, which can be used to induce apoptosis in the cells with high levels Fas expression.
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分类号：R3(基础医学)
资助基金：江苏省自然科学基金(BK99149)江苏省重大项目(BJ98025)
论文发表日期：2001-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：9( 13-21 )