Estabishment of A Human Liver Cancer Cell Line Transfected with IL-2 cDNA and Its Biologic Activity
孙跃明
王学浩
杜竞辉
1.Department of Hepatobiliary Surgery,the First Affiliated Hospital of Nanjing Medical University,2.Department of Hepatobiliary Surgery,the First Affiliated Hospital of Nanjing Medical University,3.Department of Hepatobiliary Surgery,the First Affiliated Hospital of Nanjing Medical University,
摘要：Objective To obtain IL-2 gene transfected human liver cancer cells and study IL-2 expression and its biologic activity in vivo. Methods Human liver cancer cells SMMC-7721 were cocultured with recombinant retroviral vector LNC-IL-2,and screening was performed in G418 medium.The exogenous IL-2 cDNA at the DNA,RNA,and protein levels were determined by using dot hybridization,PR-PCR and MTT methods respectively.The tumorigenesis and antitumorigenesis of the screened liver cancer cell with subcutaneous injection in nude mice were observed. Results and Conclusion The IL-2 cDNA was successfully integrated into SMMC-7721 cell genomic DNA and continuously expressed for more than 88 days.Subcutaneous vaccination of the nude mice with transfected cells revealed an obvious suppression of its tumorigenicity,and could induce antitumor activity in vivo.
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分类号：R73(肿瘤学)
论文发表日期：2001-07-02
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：3( 96-97,104 )