Regulation of Interleukin-6 Production by Cultured Human Thyrocytes
段宇
刘超
武晓泓
刘翠萍
覃又文
蒋须勤
1.Department of Endocrinology,the First Affiliated Hospital of Nanjing Medical University,Nanjing Jiangsu 210029,P.R.China2.Department of Endocrinology,the First Affiliated Hospital of Nanjing Medical University,Nanjing Jiangsu 210029,P.R.China3.Department of Endocrinology,the First Affiliated Hospital of Nanjing Medical University,Nanjing Jiangsu 210029,P.R.China4.Department of Endocrinology,the First Affiliated Hospital of Nanjing Medical University,Nanjing Jiangsu 210029,P.R.China5.Department of Endocrinology,the First Affiliated Hospital of Nanjing Medical University,Nanjing Jiangsu 210029,P.R.China6.Department of Endocrinology,the First Affiliated Hospital of Nanjing Medical University,Nanjing Jiangsu 210029,P.R.China
摘要：Objective To study the modulation of interleukin-6 (IL-6) generation from human thyroid cells.MethodsThyroid tissues were obtained at surgery from patients with Graves disease.IL-6 in the supernatant of cultured thyrocytes was detected with ultra-sensitive ELISA.mRNA was extracted respectively from primary human cultured thyroidal cells under stimulated and unstimulated conditions,and semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) technique was used for produce high concentration IL-6.Thyrotropin (TSH,103mU/L),tumor necrosis factor-α(TNF-α,10-1 U/ml),interleukin-1(IL-1,10 U/ml) and adrenaline(10-5mol/L) significantly increased IL-6 levels in the supernatant of cultured thyroid cells,whereas dexamethasone (10 mmol/L) markedly suppressed IL-6 release from thyrocytes.NaI of 10-4 mol/L results,at a concentration of 10-103 mmol/L,dexamethasone could gradually suppress IL-6 gene expression on human thyrocytes,while TNF-α could obviously stimulate the production of IL-6 mRNA in the range of 10-1-102 U/ml.On the level of 102 U/ml,IL-1 increased the expression of IL-6,but the same effect could not be shown when IL-1 was 10-1 or 10 U/ml.Sodium iodine (NaI) could not affect IL-6 gene from the level of 10-8 to 10-4 mol/L.ConclusionIL-6 produced by thyrocytes might be regulated by many factors that modulate thyroid functions in vitro as well as in vivo.``
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分类号：R58(内分泌腺疾病及代谢病)
资助基金：江苏省自然科学基金(BK95080314)
论文发表日期：2002-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：4( 18-21 )
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