Expression of Inositol 1,4, 5-trisphosphate Receptor mRNA in Myocardium of Spontaneous Hypertension Rats and Cultured Vascular Smooth Muscle Cells of Rats
刘乃丰
张寄南
耿茜
杨笛
董莉
马文珠
Division of Cardiology, Zhongda Hospital, Southeast University, Nanjing 210009
摘要：Objective To investigate expression of inositol 1,4,5-trisphosphate receptor ( IP3R) mRNA on sacroplasmic reticular in myocardium of spontaneous hypertension rats ( SHRs) and cultured vascular smooth muscle cells (VSMC) of rats and effects of perindopril and urapidil on them. Methods SHRs were orally given perindopril (1. 0 mg@ kg-1 @ d-1) or urapidil (15 mg@kg-1 @ d-1) for 24 weeks, respectively. Expression of IP3R mRNA was examined by semi-quantitatwe reverse transcription polymers chain reaction ( RT-PCR ) using three oligonuclotide primers for each subtype of IP3R with β-actin as internal label. Results All subtypes of IP3R were expressed in myocardium of SHR, WKY and cultured VSMC. Expression of IPsR mRNA in left ventricle of SHR was markedly enhanced. Urapidil could down-regulate expression of IP3R- I and IP3R- iⅢ , perindopril slightly increased expression of IP3R- Ⅱ and decreased expression of IP3R- I and IP3R- Ⅲ in myocardium of SHR. Conclusion Our results suggest that expression of IP3R mRNA in cardiovascular system could be regulated by urapidil and perindopril.
机标关键词：
分类号：R3(基础医学)
论文发表日期：2002-04-02
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：5( 75-79 )
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