A Novel Antisense Oligodeoxynucleotide Targeting Caspase-3 mRNA Prevents PC-12 Cell Apoptosis Induced by Aβ25-35
王亚利
宋天保
王中卫
许琨
1.Department of Histology and Embryology, Medical College of Xi'an Jiaotong University, Xi'an 710061 ,P. R. China2.Department of Histology and Embryology, Medical College of Xi'an Jiaotong University, Xi'an 710061 ,P. R. China3.Department of Histology and Embryology, Medical College of Xi'an Jiaotong University, Xi'an 710061 ,P. R. China4.Department of Histology and Embryology, Medical College of Xi'an Jiaotong University, Xi'an 710061 ,P. R. China
摘要：Objective To design and synthesize antisense oligodeoxynucleotides ( ASODNs )targeting caspase-3 mRNA and to screen the effectwe ASODNs to prevent PC-12 cells alwptosis in-dnced by Aβ25-35. Methods 17-18-mer ASODNs targeting different regions of caspase- 3 mRNAwere designed and transferred into PC-12 cells with Lipofectimine and followed by induction withAβ25-35. The effective ASODN preventing PC-1 2 cells from apoptosis was screened according to the re-sults of Flow Cytometry ( FCM ) analysis with annexin V -FITC/Propidium Iodide (PI) dual staining.Results FCM analysis by annexin V /PI staining showed that the ratio of apoptotic cells decreasedmore significantly than other ODNs after the ASODN2 (-GTT GTT GTC CAT GGT CAC-) ( P ＜0. 05), which targets bases - 6-12 of caspase- 3 mRNA and was transferred into PC-12 cells , thenthe cells were exposed to Aβ25-35. Apoptotic cells transferred with ASODN2 treated with Aβ25-35 werediffererzt from Aβ25-35 group without statistical significance. Conclusion Caspase-3 plays an im-portant role in the PC-12 cells apoptosis treated with Aβ25-35. The ASODN2 aimed at caspase-3 mRNAwas identified as an effective fragment to prevent the PC-12 cells from apoptosis treated with Aβ25-35.
机标关键词：
分类号：R3(基础医学)
资助基金：国家自然科学基金(989m61)
论文发表日期：2002-07-02
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：6( 97-102 )
英文信息