Effects of Nerve Growth Factor on Bcl-2 Protein after Spinal Cord Injury in Rats
汤长华
曹晓建
王道新
1.Department of Orthopedics, the First Affiliated Hospital of NJMU, Nanjing 210029,P. R. China2.Department of Orthopedics, the First Affiliated Hospital of NJMU, Nanjing 210029,P. R. China3.Department of Orthopedics, the First Affiliated Hospital of NJMU, Nanjing 210029,P. R. China
摘要：Objective To explore the protective mechanisms of nerve growth factor( NGF) ou spinal cord injury(SCI) and provide theoretical basis for its clinical application. MethodsThe SCI of Wistar rats was done by Allens weight dropping way by a 10 g × 2.5 cm impact on theposterior of spinal cord T8 NGF ( 3 g/L, 20d) or normal saline was injected to treatment group ratsthrough catheter into subarachnoid space at 0,2,4,8,12 and 24 h after SCI. The expression of bcl-2 protein levels in rat spinal cord was detected by immunohistoclemistry. Results The strong expres-sion sequence of bcl-2 protein was found in spinal cord of normal rat group. The levels of bcl-2 pro-tein after SCI in NGF treatment group increased more significantly than those in normal saline treatmentgroup (P＜0. 01). Conclusion NGF could protect injured spinal cord by stimulating bcl-2 pro-tein expression and suppressing apoptosis after SCI.
机标关键词：
分类号：R3(基础医学)
资助基金：国家自然科学基金(39800176)
论文发表日期：2002-07-02
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：3( 112-114 )
英文信息