The Chondrogenic Potential of Rabbit Bone-marrow-derived Mesenchymal Stem Cells (MSCs) As-sayed under the Influence of Growth Factors
尹战海
刘淼
王金堂
张璟
曹峻岭
郑钧
1.the First Hospital of Xi'an Jiaotong Univer-sity2.the First Hospital of Xi'an Jiaotong Univer-sity3.the First Hospital of Xi'an Jiaotong Univer-sity4.5.6.the First Hospital of Xi'an Jiaotong Univer-sity
摘要：Objective To establish a method of reducing rabbit bone marrow mesenchymalstem cells (MSCs) to express chondrocytic phenotype. Methods MSCs were seperated from bonemarrow ertracted from the iliac of New Zealand rabbit. TGF-β1, IGF- I and Vitamin C were appliedinto culture medium to indntee proliferation and transformation of MSCs. Colony forming efficiency( CFE) was measured to reflect cell proliferation rate; procollagen al ( Ⅱ )mRNA in cells was detectedby RT- PCR ; ultrastructure of cells was observed under scanning electronic microscope ( SEM ) ; alklinephosphatase(ALP ) in cullure medium wts also detected. Results Under the influence of TGF-β1,IGF-1 and Vitamine C, CFE of MSCs rose from control level of 3/106 to 21/106, expression ofarticular cartilage specific procollagen al ( Ⅱ )mRNA by cells was promoted; At the same time, floccu-lent matrixes synthesized were observed between connecting polygonal MSCs. ALP in culture medium de-clined to control level with culture time. Conclusion Expression of chondrocytic phenotype byMSCs could be induced by the synergistic action of TGF-β1, IGF- I and Vitamine C. Growth factor-intduced MSCs could be a good cell source for tissue-engineered cartilage.
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分类号：R3(基础医学)
论文发表日期：2002-07-02
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：6( 121-126 )
英文信息