Relationship between Vasodilation Effect of Anisodamine and Endothelial Target for Acetylcholine on Microvessels in Rat
鲍颖霞1
李庆平1
汪海2
1.Department of Pharmacology, Nanjing Medical University, Nanjing,2100292.Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Beijing 100850,P.R.China
摘要：Objective To study the effects of anisodamine on microvessels and its relatwn-ship with endothelium target for acetylcholine (ETA). Methods Norepinephrine precontracted mesen-teric vascular beds in rat were used to determine changes of tension of vessels in response to anisodamineafter removal of endothelium , coincubated with L-NAME, nitric oxide synthase (NOS) inhibitor, in-domethacin , cyclo-oxygenase inhibitor and glibenclamide , ATP-dependent K+ channels (K ATP ) inhibitor.Results Anisodamine produced a vasodilation effect on mesenteric vascular beds, which was remark-ably inhibited after removal of endothelium (P＜ 0.01). The vasodilation effect of anisodamine was al-so significantly inhibited after coincubated with indomethacin (1 μmol/ L), NG-nitro-L-arginine methylester ( L- NAME, 100 μrmol / L) and glibenclamide (1 μmol/L, P＜0. 01, vs control). Conclu-sion The vasodilation effect of ani.sodamine on microvessels was mediated by ETA. This is related toprostacyclin , endothelium derived relaxing factor (EDRF) and KATP besides M, a-receptor on vascularmooth muscle cell (VSMC) and calcium-antagonistic effects.
机标关键词：
分类号：R3(基础医学)
资助基金：国家重点基础研究发展计划（973计划）(G1998051112)
论文发表日期：2002-10-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：4( 137-140 )
英文信息