Association Studies of 3 Candidate Genes with Type 2 Diabetes Mellitus in a Chinese Population
鲁一兵1
缪珩1
王华2
何戎华2
马立隽3
金卫新3
华子春3
1.Department of Endocrinology, the Second Affiliated Hospital of Nanjing Medical University, Nanjing,2100112.Department of Endocrinology, the First Affiliated Hospital of Nanjing Medical University,Nanjing,2100293.State Laboratory Pharmaceutical Biotechnology, Nanjing University, Nanjing,210008,P. R. China
摘要：Objectives To explore the relationship between the polymorphisms of the select-ed short tandem repeats (STRs) of the candidate genes and type 2 diabetes mellitus (DM) in a Chinesepopulation, the role of genetic and environmental factors in the development of type 2 diabetes. Meth-ods STRs including D11S916 of uncoupling protein 3 (UCP3) gene,binucleotide repeat (CA). with-in intron 6 [HSLi6 (CA)n] of hormone- sensitive lipase(HSL) gene and D20S501 of protein tyrosinephosphatase- 1B (PTP-1B) gene polymorphisms were detected by polymerase chain reaction (PCR) , poly-acrylamiie gel electrophoresis and silver staining in 106 patients with type 2 DM and 102 control sub-jects. Results The allele distribution of UCP3 and HSL gene differed significantly between patientswith type 2 diabetes and control subjects (χ2 = 26. 12, P＜0.005; χ2=10. 33, P＜0. 005, respec-tively). For UCP3 and HSL gene,the frequencies of alleles A6,A7 ,A8 and allele B9 were much high-er in diabetic patients than in control subjects (0. 090 vs 0. 020,P＜0. 005; 0. 109 vs 0. 015,P＜0. 005; 0. 033 υs 0. 000,P＜0.05; 0. 033 υs 0. 005,P＜0. 05,respectively),while the fre-quencies of allele A1 and allele B5 were lower in diabetic patients than in control subjects (0. 090 vs0. 206,P＜0. 005; 0. 057 vs 0. 118,P＜0. 05,respectively). At D20S501 locus,The allele dis-tribution of PTP-1B gene had no significant difference in two groups (χ2=3. 77 ,P＞0. 05). Multi-variate logistic regression analysis showed positive correlation between alleles A 6,A 7 of UCP3 gene,sys-tolic blood pressure , apolipoprotein B , lipoprotein (a) and type 2 diabetes. Conclusion Our datashow that D11S916 of UCP3 gene and HSLi6 (CA), of HSL gene polymorphisms are associated withtype 2 diabetes in Chinese suggesting that UCP3 and HSL might represent susceptibility genes for type 2diabetes. D20S501 of PTP-1B gene polymorphism isnot associated uith type 2 diabetes in Chinese. AllelesA6, A7 of UCP3 gene, systolic blood pressure,apolipoprotein B, lipoprotein (a) may play same rolein the development of type 2 diabetes.
机标关键词：
分类号：R3(基础医学)
资助基金：国家自然科学基金(39500072)江苏省教育厅自然科学研究项目(99KJB320002)
论文发表日期：2002-10-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：7( 149-155 )
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