Establishment of Allergic Airway Inflammation Model in Late- phase Response of Sprague- Dawley Rats
朱敏敏
傅诚章
周钦海
1.Department of Anesthesiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029 ,P. R. China2.Department of Anesthesiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029 ,P. R. China3.Department of Anesthesiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029 ,P. R. China
摘要：Objective To establish allergic airway inflammation model in late-phase airwayreaction of Sprague-Dawley (SD) rats. Methods Thirty-six SD rats were randomly divided intothree groups: control group (Group Ⅰ),single challenge group (Group Ⅱ),consecutive challenge group(Group Ⅲ). The rats in Group Ⅱ and Group Ⅲ were sensitized twice by injection of ovalbumin (OA) to-gether with aluminum hydroxide and Bordetella pertussis as adjuvants, followed by challenge withaerosolized OA for 20 min once in Group Ⅱ or one time on each day for one week in Group Ⅲ . Therats in Group Ⅰ received 0.9 % saline by injection and inhalation. Results Conpared uith groupⅠ , there were positive symptoms observed in the group Ⅱ and group Ⅲ; the amount of total leucocytesand eosinophil percentage in brochoalveolar lauage fluid (BALF) significantly increased (P＜0.05 orP ＜0.01 respectively) in Group Ⅱ or Ⅲ; histopathologic changes of lung showed acute allergic inflam-mation changes in Group Ⅱ : Disrupted epithelium damaged subepithelial structure and eosinophil infiltra-tion the in the airway wall. As for the Group Ⅲ , there were allergen-induced characteristic features ofchronic allergic airways inflammation: hypertrophy and hyperplasia of bronchial smooth muscle, gobletcell hyperplasia , basement membrane thickening, eosinophil infiltration, edema. Conclusion The mod-el of allergic airway inflammation in late-phase response of SD rats was successfully established by OAsensitization (twice) and consecutive challenge.
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分类号：R3(基础医学)
资助基金：江苏省自然科学基金(BK2001160)
论文发表日期：2002-10-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：5( 163-167 )
英文信息