Analysis of the Relationship between Genome Diversity and Adult Survive Rate of Botryllus Schlosseri by AFLP
FENG Xiao-rong
ZHU Jian-zhong
Deng Feng-jiao
Jacob Douek
Baruch Rinkevich
摘要：Objective: The self-cross colonial prochordate, Botryllus schlosseri ( B. schlosseri) occupy a key phylogenetic position in the evolution of vertebrates. To clarify the relationship of genome diversity and survive rate, five generations of B.schlosseri was investigated by amplified fragment length polymorphism (AFLP). Methods:AFLP markersare extremely sensitive to even smell sequence variation, using PCR and high-resolution electrophoresis to examine restriction fragments. Results: AFLP polymorphism was high in the parent and lower in its F1, F2, F3 and F4. Each primer combination generated from 80 to more than 120 bands, of which average 25.85% poiymorphic loci in parent, 15.79% polymorphic among F1, 9.16% and 5.58% in F2,F3. The AFLP markers were transmitted from F1 to F2, F3 and F4 and inherited, segregated in expected Mendelian ratio. However, some of the markers were lost in F2, F3 and F4 while it disappeared in their mother. In addition, gene mutation-new loci and lost loci among F1, F2, F3 and F4 were observed. These special fragments were cloned and sequenced. Then, the genomic DNA was analyzed by Southem hybridization with the probes from these specific fragments and the mechanism of gene mutation was clarified. Conclusion:The se results suggest that there are high frequency of polymorphic loci and mutation in genome of B.schlosseri. Gene deletion or iow diversity may be the reason for high rate of death of the offspring of inbred laboratory-reared strains.
机标关键词：
分类号：Q81(生物工程学(生物技术))
论文发表日期：2004-03-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：11( 67-77 )
英文信息