The Special Feature of Calponin on Myosins Phosphorylated by MLCK and PKA Respectively
Jingxian Yang
Shuyuan Li
Guozhu Han
Yuan Lin
Jingfeng Zhang
摘要：Objective: To reveal the special feature of calponin (CaP) on myosins of different states. Methods: Myosin phosphorylation determination, myosin Mg2 +-ATPase measurement and protein binding assay were used in this study. The lowest CaP/myosin ratio used in the assay was 1/10000(mol/mol), which was 10 thousands-fold lower than the CaP/myosin ratio used in previous studies. Results: In the absence of actin, micro-amount of calponin (MAC) stimulated the Mg2+-ATPase activities of myosin in different states slightly but significantly; and more importantly, MAC significantly increased the precipitations of unphosphorylated myosin, Ca2 + -de-pendently and independently phosphorylated myosins by MLCK but not the myosin phosphorylated by PKA. Conclusion: MAC has a high efficient and selective effect on myosin in the absence of actin.
机标关键词：
分类号：R3(基础医学)
资助基金：国家自然科学基金(30070203)
论文发表日期：2005-05-02
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：5( 111-115 )
英文信息