Molecular cloning of human heat shock protein 27 and study of its protective effects on oxidative damage in rat cardiomyocte H9c2
Li Liu
Xiaojin Zhang
Surong Jiang
Xiang Gao
Guoxain Ding
Yunlin Cheng
摘要：Objective: To clone human cardiac heat shock protein 27(HSP27) gene and to determine the effects of HSP27 on the oxidative stress in rat cardiomyocyte cell line H9c2. Methods: Full length of HSP27 cDNA which got by RT-PCR was constructed into pCDNA3.1 + . The recombinant was transfected into rat cardiomyocyte cell line H9c2 and the stable transfection cell line was selected by G418. Then we observe the effects of HSP27 over-expression on LDH release and apoptosis induced H2O2 in H9c2. Results:1000 μmol/L H2O2 in HSP27 over-expression group and wild type group were 0.396 ± 0.017 vs. 0.390 ± 0.009 ( p ＞ 0.05 ), 0.437 ± 0.014 vs. 0.416 ± 0.015 ( P ＜ 0.05), 0.471 ± 0.018 vs. 0.417 ± 0.009 ( P ＜ 0.001), 0. 505 ± 0.030 vs. 0. 657 ± 0.022 ( P ＜ 0.001),and wild type group were (10.693 ± 1.122) % vs. (4.027 ± 1.628 )% ( P ＜ 0.01). Conclusion: We cloned and constructed human cardiac HSP27 gene successfully, and over-expression of human HSP27 could inhibit oxidative damage significantly in H9c2.
机标关键词：
分类号：R5(内科学)
资助基金：教学改革项目(CX2003018)
论文发表日期：2005-07-02
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：4( 187-190 )
英文信息