Reactive oxygen species in paraventricular nucleus involved in cardiac sympathetic afferent reflex in rats
Feng Zhang
Yang Yu
Ying Zhang
Yingchun Li
Luqing Zhang
Lingling Fan
Yingya Gao
Guoqing Zhu
摘要：Objective: The present study was designed to determine if reactive oxygen species (ROS) in the paraventricularnucleus (PVN) were involved in modulating cardiac sympathetic afferent reflex (CSAR) in anesthetized rats. Methods: Malondialdehyde(MDA), the end product of lipid peroxidation, in the PVN, was determined by thiobarbituric acid (TBA) spectrometric method. Renalsympathetic nerve activity (RSNA) and arterial pressure were recorded in sinoaortic-denervated and cervical-vagotomized rats. The CSARwas evaluated by the response of the RSNA evoked by epicardial application of bradykinin (BK, 0.4 μg). Results: The MDA in the PVNwas significantly increased after epicardial application of BK compared with control (2.0 + 0.3 vs 0.8 + 0.1 nmol/mg protein, P ＜ 0.01 ).Microinjectionof a superoxide anion scavenger, tiron (20 nmol) into the PVN significantly inhibited the CSAR evoked by BK (12.3 ± 1.9vs 4.2+ 1.2%, P ＜ 0.01) and decreased MDA level (1.9±0.3 vs 0.6+0.1 nmol/mg protein, P ＜0.01) compared with control.Conclusion: The ROS in the PVN is involved in modulating the CSAR in rats.
机标关键词：
分类号：R5(内科学)
资助基金：中国科学院项目（非规范项目）(30470632)教学改革项目(NY04004)
论文发表日期：2005-11-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：5( 289-293 )
英文信息