RhoA/ROCK signaling in chondrogenic differentiation of MSCs induced by TGF-β1
Liang Xu
Shuhua Yang
Hongtao Tian
摘要：Objective: To investigate the role of RhoA/ROCK in the process of chondrogenic differentiation of MSCs in vitro induced by TGF-β1. Methods: MSCs were isolated from rat bone marrow, cultured and passaged. The cultured MSCs at the 3rd passage were induced to differentiate into chondrocytes in induction medium containing TGF-β1, the expressions of RhoA and ROCK1/2 in the induced MSCs cells were detected by Western-blot and RT-PCR. Results: In parallel to chondrogenic marker gene expressions of collagen Ⅱ and aggrecan, ROCK inhibition by Y27632 in these cells caused a significant decrease in mRNA level of collagen Ⅱ. Conclusion: The results suggest that RhoA/ROCK pathway may play an important and complex role in regulation of chondrogenic differentiation and gene expression.
机标关键词：
分类号：R3(基础医学)
资助基金：国家自然科学基金(30471753)
论文发表日期：2006-11-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：5( 346-350 )
英文信息