Studies on the apoptosis of autonomic neuron in streptozotocin-induced diabetic rats
Qinglin Lou
Rongwen Bian
Tao Peng
Xiaojun Ouyang
Hui Xia
Liubao Gu
Yongzhen Mo
摘要：Objective: The aim of the study was to investigate apoptosis of the autonomic neuron in streptozotocin-induced diabetic rats, and observe the effect of intervention with nerve growth factor (NGF) on the apoptosis. Methods: A total of 29 male Sprague-Dawley rats were divided into three groups, i.e. normal control (NC, n = 12), untreated diabetic (DM, n =9) and diabetic treated with NGF daily of 500 μg/kg for 30 days(DM+NGF, n = 8). The diabetic rat models were produced by intraperitoneal injection with streptozotocin(65 mg/kg). Over 3 months since the diabetes were setup, the superior cervical sympathetic ganglions(SCG) and the celiac ganglions(CG) were removed and fixed with 10% paraformaldchyde. Apoptosis was measured using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling(TUNEL). Apoptotic index (AI) was calculated by computer image analysis system. Results: The AI of SCG and CG in DM and DM+NGF group were significant higher than those of NC group (P ＜ 0.001) respectively. There was no difference of AI of SCG and CG between DM group and DM+NGF group (both P ＞ 0.05). Conclusion: Neuron apoptosis may contribute to the pathophysiology of diabetic autonomic neuropathy and NGF can not prevent the apoptosis of autonomic neuron in diabetic rats.
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分类号：R5(内科学)
论文发表日期：2006-11-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：4( 383-386 )
英文信息