Detecting katG Drug Resistant Genetic Mutation among pneumoconiosis patients complicated with tuberculosis in Mycobacterium tuberculosis L-forms by PCR-SSCP
Jun Lu
Zhao Zheng
Song Ye
Chaopin Li
摘要：Objective: To study the relationship between mutation in the katG gene and drug resistance of INH in Mycobacterium tuberculosis L-forms among patients of pneumoconiosis complicated with pulmonary tuberculosis, and to explore the clinical application of PCR-SSCP. Methods:A total of 52 clinical isolated strains of Mycobacterium tuberculosis L-forms were collected. Mutation in the katG genes was detected by PCR-SSCP and traditional antimicrobial susceptibility test (AST). Results:The results by AST showed that there were 40 persisters in 52 clinical isolated strains. The drug resistant rate was 76.92%(40/52),and the gene mutation rate of katG was 57.70% (30/52)by.PCR-SSCP, the difference was no quite significance (χ2 = 2.8507, P ＞0.05). The coincidence rate of two methods was 75.00% (30/40). Conclusion: The detectionrate of katG resistant strains in Mycobacterium tuberculosis L-forms was high by PCR-SSCP. The combined application of PCR-SSCP and traditional antimicrobial susceptibility test can improve the detecting rate.
机标关键词：
分类号：R9(药学)
资助基金：教学改革项目(200537)
论文发表日期：2006-11-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：3( 392-394 )
英文信息