Selection of DMA aptamer that specific binding human carcinoembryonic antigen in vitro
Lifeng Wang
Baorui Liu
Haitao Yin
Jia Wei
Xiaoping Qian
Lixia Yu
摘要：Objective:To select the specific aptamer of carcinoembryonic antigen (CEA), one of the most attractive molecule for cancer target therapy and imaging. Methods: Seven rounds in vitro selection were performed against the purified CEA protein. Ligand-mediated target purification and Co-immunoprecipitation were adopted to verify the specific binding of the aptamer to the purified and native protein separately. Results:The CEA-specific aptamer which can bind both the purified and native protein with the high specificity was obtained. Conclusion:This is the first time the CEA specific apatmer was produced. The results in this study provides the preliminary evidence for further investigation and application of CEA-aptamer in the future.
机标关键词：
分类号：R3(基础医学)
资助基金：国家自然科学基金(30670958)
论文发表日期：2007-09-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：5( 277-281 )
英文信息