Inhibition of cell proliferation by siRNA targeting hPRLR in breast cancer MCF-7 cell line
Mei Pan
Qinjun Wei
Fang Cao
Yajie Lu
Yibao Zhu
Yongqian Shu
Xin Cao
摘要：Objective:To study the inhibition of proliferation of breast cancer by small interfering RNA(siRNA)targeting human prolactin (hPRLR)and the underlying mechanisms.Methods:The siRNA targeting hPRLR was chemically synthesized and transfected into MCF-7 cells.the expression of hPRLR was analyzed by real-time quantitive PCR,cell growth inhibition was measured with MTT assay,cell cycle of the transfected cells Was examined by flow cytometry,meanwhile,expression of cyclin D1 Was tested by semi-quantitative RT-PCR.Results:24 h after transfection with 100 nmol/L siRNA-PRLR,the expression of hPRLR mRNA was suppressed by 65%,cells in G1 phase increased,but cells in S phase decreased.Down regulated hPRLR expression exhibited significant inhibition in cell proliferation.And the expression of cyclin D1 was down regulated.Conclusion:The results indicate that siRNA-hPRLR iS a useful tool for silencing hPRLR expression and inhibiting cell proliferation in breast cancer MCF-7 cell line,and it may be a possible new approach for breast cancer gene therapy.
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分类号：R73(肿瘤学)
资助基金：教学改革项目(CX2002004)
论文发表日期：2007-11-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：5( 372-376 )
英文信息