Comparison between synthetic retinoid CD437 and acitretin inhibiting melanoma A375 cell in vitro
Jianwen Ren
Zhenhui Peng
Min Pan
Birong Guo
Yan Liu
Xianglan Wang
摘要：Objective: To investigate the effects of synthetic retinoid CD437 and acitretin on cell proliferation, apoptosis, cycle arrest and Bax/Bcl-2 protein expression of melanoma A375 cell. Methods:MTT assay was used to determine the anti-proliferative effects of CD437 and acitretin on melanoma A375 cell. Flow cytometry was performed to investigate the influence of CD437 and acitretin on cell cycle and cell apoptosis. SABC immunocytochemistry was employed for detection of Bax/bcl-2 protein expressions. Results:10-5 mol/L CD437 was more effective than acitretin in inhibiting proliferation and inducing apoptosis of A375 cell after 24 h treatment, growth inhibiting ratio and apoptosis ratio(58.6%vs43.25% and 28.03%vs17.13%, P ＜ 0.05 respectively). CD437 promoted G0/G1 arrest in melanoma A375 cell, however acitretin could not. CD437 and acitretin could up-regulate the expression of Bax protein and downregulate the expression of bcl-2 protein(P＜ 0.05). Conclusion:CD437 is more effective than acitretin in inhibiting proliferation and inducing apoptosis and cycle arrest on A375 cell. CD437 may have more potentialities than acitretin for subsidiary treatment of melanoma. Mitochondrial apoptosis pathway is partially involved in two drugs inducing apoptosis on A375 cell.
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分类号：R3(基础医学)
资助基金：国家自然科学基金(30371295)
论文发表日期：2008-03-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：5( 87-91 )
英文信息