The effect of Nifedipine on the expression of type I collagen in gingival fibroblasts
Bei Li
Weibin Sun
Yong Ji
摘要：Objective:To investigate the effect of nifedipine(calciumchannel blocker) on the expression of collagen in gingival fibroblasts invitro, Methods:Primarily gingival fibroblasts were cultured and incubated with various concentrations of nifedipine(108 μg/L, 360μg/L and 1200 μg/L)for 5 days. Gingival fibroblasts were primarily cultured derived from nifedipine responders and non-responders in the presence of 360 μg/L nifedipine. Enzyme-linked immunosorbent assay was used to evaluate the amount of type Ⅰ collagen. Cell proliferation was measured by cell counting with evaluating MTT value. Results:The expressions of collagen and cell proliferation were significantly different among the high concentration groups and the others on the fifth day, especially higher in 360 μg/L and 1200 μg/L groups and also different among nifedipine responders and non-responders. Conclusion:The expression of collagen and cell proliferation may be concerned with the biological mechanism for gingival overgrowth.
机标关键词：
分类号：R78(口腔科学)
资助基金：江苏省自然科学基金(BK2006172)
论文发表日期：2008-03-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：4( 92-95 )
英文信息