Expression of Beclin 1 in non small cell lung cancer and its clinical significance
Quan Liu
Jianjun Wang
Yongcheng Pan
Lifeng Meng
Xi Zhan
摘要：Objective:To investigate the expression of autophagy related gene; Beclin1 in human non-small cell lung cancer(NSCLC) tissues. Methods:Protein expression of Beclinl was determined by immunofluorescence staining and Western Blot, mRNA expression was analyzed by RT-PCR. Results:Immunofluorescence staining revealed that the level of Beclinl expression in lung cancer was significantly lower than that in adjacent noncancerous tissues and normal tissues(expression rate 8.3%, P=0.000). The Bechnl mRNA expression in lung cancer, adjacent noncancerous tissues and normal tissues was 1.372 (± 0.475) 1.721 (± 0.521) and 1.553(± 0.554) when F=15.0, P ＜0.01. Beclinl protein expression in lung cancer, adjacent noncancerous tissues and normal tissues was 3.453(± 0.852) 5.423(±1.351) and 6.878(± 0.997) F=11.2, P ＜ 0.01. Conclusion:The protein and mRNA expression of Beclin 1 in lung cancer was much lower than those in and around cancer tissues and normal tissues, those differences having statistical significance. However in adjacent noncancerous tissues and normal tissues, the expression showed no difference.
机标关键词：
分类号：R73(肿瘤学)
论文发表日期：2008-03-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：4( 121-124 )
英文信息