Research on the effects of CD137 signaling on the function ofCD3-CD56+NK cells
Yu Zhang
Songwen Ju
Yongqian Shu
摘要：0bjective:To investigate the effects of CD137 signaling on the regulation of CD3-CD56+NK cells function.Methods:CD3-CD56+NK cells were treated with CD137 mAb or mouse IgG1 isotype control to study the effects of CD137 signaling on the function of CD3-CD56+NK cells.Cytotoxicity was measured by LDH activity in the supernatants of cell cultures; NKG2D and LFA-1 expression on CD3-CD56+NK cells were analyzed by flow cytometry.Results: CD137 was expressed on activated CD3-CD56+NK cells.The CD137 mAb enhanced the ability of CD3-CD56+NK cells to kill lung cancer cel]s(A549); Further studies revealed that the expression of NKG2D and LFA-1 was significantly increased in activated cells,and blockade of NKG2D and LFA-1 dramatically attenuated CD3-CD56+NK cytolysis of A549 cancer cells.Conclusion: CD137 signaling increases the ability of CD3-CD56+NK cells to kill cancer cells via up-regulating the expression of NKG2D and LFA-1.
机标关键词：
分类号：R5(内科学)
资助基金：国家自然科学基金(30772549)
论文发表日期：2009-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：5( 10-14 )
英文信息