Effect of hepatic ischemia-reperfusion on bile canalicular F-actin microfilaments in rats
Yiming Li
Hua Li
Jidong Liu
Hong Ji
摘要：Objective:To investigate the effect of hepatic isehemia-reperfusion(I/R) on bile canalicular F-actin microfilaments in rats. Methods: A rat model of hepatic ischemia-reperfusion was employed and the ischemia time was 35 rain. The activity of serum alanine aminotransferase (ALT), aspartate aminotransferase(AST), γ-glutamyl transferase(GGT) and the level of total bilirubin(TBIL) were measured. Changes in the bile canaliculi were observed by transmission electron microscope. The modification of F-actin microfilaments was quantified by using FITC-PhaUoidin and analyzed by confocal laser scanning microscopy imaging. Results:Modifications of F-actin staining were consistent with the observations made by transmission electron microscopy. The staining of F-actin was normal in hepatocytes before reperfusion but decreased significantly after reperfusion, and there was a marked loss of canalicular microvilli after reperfusion, which coincided with abnormal serum GGT and TBIL levels. Conclusion:Reperfusion, not short-term ischemia, induced a disruption of F-actin microfilaments and a loss of microvilli. These modifications could lead to the impaired bile secretion by damaging canaUcular contraction, and could be the main mechanisms of cholestasis after hepatic ischemia-reperfusion in rats.
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分类号：R5(内科学)
资助基金：国家自然科学基金(30571765)
论文发表日期：2009-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：6( 137-142 )
英文信息