Microdilution inhibition test of Chinese herbs to assess their effect against clinical strains of Ureaplasma urealyticum in vitro
Changtai Zhu
Chunlei Dong
Yinze Kong
Lingxia Liu
Qingsi Wu
Yuyou Yao
摘要：Objective:To explore the antibacterial effect of Chinese crude drugs against clinical strains of Ureaplasma urealyticum(UU), including eight pure herbs and three compound herbs, and determine their minimal inhibitory concentrations(MICs). Methods:Isolates were collected from clinical patients with UU infection, and cultured in UU broth. In order to test the different effects on clinical strains of UU, the assays were performed by microdilution inhibition tests, and MICs of the herbs against the clinical strains of UU were calculated. Results:The MICs of eight pure herbs against clinical strains of UU were as follows: Galla Chinensis:0.313～1.25 g/L;Fructus Forsythiae: 1.25～5.00 g/L;Cortex PheUodendri: 1.25～5.00 g/L;Radix Paeoniae Rubra: 1.25～2.50 g/L;Semen Plantaginis:2.50～10.00 g/L;Herba Lysimachiae: 5.00～20.00 g/L;Rhizoma Coptidis: 5.00～20.00g/L, Herba Houtmyniae: 10.00～20.00 g/L. The MICs of compound herbs were: Liuheji: 0.625～2.50 g/L;Bazhengsan: 5.00～20.00 g/L;Wulinsan:2.50～20.0 g/L.Conclusion:Galla Chinensis, Fructus Forsythiae, Cortex Pheilodendfim, Radix Paeoniae Rubra, and Semen Plantaginis, exerted the stronger antibacterial effect against clinical strains of UU, whereas Herba Lysimachiae, Rhizoma Coptidis and Herba Houttuyniae, had relatively weaker activity against UU. Compound herbs,Bazhengsan and Wulinsan, and particularly Liuheji, also had antibacterial effects against UU. Further studies of the effects and mechanisms of action of Chinese crude drugs against UU infections are worthwhile.
机标关键词：in vitroUreaplasma urealyticumChinese herbsantibacterial effectMICsinhibition testsdifferentIn order
分类号：R5(内科学)
论文发表日期：2009-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：3( 143-145 )
英文信息