Effects of C-reactive protein on the expression of OX40 ligand in mouse aorta endothelial cells
Fei Wang
Shushu Zhu
Xuefu Han
Jindan Xu
Jinnan Zhang
Di Yang
摘要：Objective: To investigate the expression of OX40 ligand(OX40L) on C-reactive protein(CRP)-triggered mouse aorta endothelial cells (MAECs) in vitro. Methods: MAECs from aorta were isolated by digestion with collagenase type Ⅱ. The cell growth was confirmed by morphological characteristics and the immunological marker, factor Ⅷ(or Willebrand factor, vWF). The expression of OX40L by MAECs was detected by RT-PCR and western blot after incubating with 100 μg/ml CRP for 48 hours. Results: Twenty-day cultures of MAECs formed confluent monolayer of a cobblestone pattern. RT-PCR and western blot assay showed that the level of OX40L expression in MAECs receiving CRP treatment was higher than control. Conclusion: A reliable method is described to isolate and propagate MAECs. CRP upregulates OX40L expression in MAECs.
机标关键词：endothelial cellsmorphological characteristicswestern blotOX40LRT-PCRCRPcell growthtreatment
分类号：R5(内科学)
资助基金：国家自然科学基金(30770894)Medical Foundation key scholar ofJiangsu Province(RC2007037)
论文发表日期：2009-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：4( 247-250 )
英文信息