Inhibition of cellular proliferation and induction of apoptosis in human esophageal carcinoma cell lines by extracts of Dioscorea bulbifera L and Chinese Angelica
摘要：Objective: To investigate the antiproliferative and apoptogenic activities of polysaccharides extracted from Dioscorea bulbifera L (DBP) and Chinese Angelica (CAP) and a 3:2 mixture of these compounds (DCCP) on two esophageal carcinoma cell lines, EC9706 and Ecal09. Methods: A MTT assay was used to detect the effects of DBP (10 μg/ml and 100 μg/ml), DCCP(17 μglml and 170 μg/ ml) and CAP (10 μg/ml and 100 μg/ml) on the proliferation of EC9706 and Ecal09 cells. DNA content analysis by flow cytometry was used to determine the cell cycle distribution, and Annexin V-FITC/PI stained fluorescence-activated cell sorter (FACS) was used to detect the apotosis rate of treated cells. Western blots were used to examine protein levels. Results: DBP and DCCP strongly inhibited the proliferation and viability of both the EC9706 and Ecal09 cells. CAP enhanced the effects of DBP. DCCP primarily arrested the EC9706 and Ecal09 cells at the Gl phase of the cell cycle. DCCP induced apoptosis in both esophageal carcinoma cell lines, and reduced the expression of plκBa and Bcl-2 proteins. Conclusion: DCCP triggered apoptosis in esophageal carcinoma cells by inhibiting the NF-κB signaling pathway.
机标关键词：Chinesecell linesesophageal carcinoma cellsEC9706DCCPcell cycle distributioncontent analysisflow cytometry
分类号：R5(内科学)
论文发表日期：2009-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：5( 398-402 )
英文信息