The preparation and characterization of folate-conjugated human serum albumin magnetic cisplatin nanoparticles
Daozhen Chen
Qiusha Tang
Wenqun Xue
Jingying Xiang
Li Zhang
Xinru Wang
摘要：Objective: Nanoparticles are becoming an important method of targeted drug delivery. To evaluate the importance of folate-conjugated human serum albumin (HAS) magnetic nanoparticles (Folate-CDDP/HAS MNP),we prepared drug-loaded Folate-CDDP/HAS MNPs and characterized their features. Methods: First,folate was conjugated with HAS under the effect of a condensing agent,and the conjugating rate was evaluated by a colorimetric method using 2,4,6 - trinitrobenzene sulfonic acid. Second,under N2 gas,Fe3O4 magnetic nanomaterials were prepared and characterized by using transmission electron microscopy (TEM),SEM-EDS and X-ray diffraction (XRD). Finally,Folate-CDDP/HAS MNP was prepared by using a solvent evaporation technique.TEM was used to observe particle morphology. The particle size and distribution of the prepared complexes were determined by a Laser particle size analyzer. Drug loading volume and drug release were investigated by a high performance liquid chromatography method (HPLC) in vitro. Results: We successfully prepared folate-conjugated HAS and its conjugating rate was 27.26 μg/mg. Under TEM,Fe3O4 magnetic nanoparticles were highly electron density and had an even size distribution in the range of 10-20 nm. It was confirmed by SEM-EDS and XRD that Fe3O4 magnetic nanoparticles had been successfully prepared. Under TEM,drug-loaded magnetic nanoparticles were observed,which had a round shape,similar uniform size and smooth surface. Their average size was 79 nm which was determined by laser scattering,and they exhibited magnetic responsiveness. Encapsulation efficiency was 89.75% and effective drug loading was calculated to be 15.25%. The release results in vitro showed that the half release time (t1/2) of cisplatin in cisplatin Solution and Folate-CDDP/HAS MNP was 65 min and 24 h respectively,which indicated that microspheres had an obvious effect of sustained-release. Conclusion: Folate-CDDP/HAS MNPs were prepared successfully. The preparation process and related characteristics data provided a foundation for further study,including the mechanism of the nanoparticles distribution in vivo and their intake by tumor cells.
机标关键词：cisplatinhuman serum albuminmagnetic nanoparticleshigh performance liquid chromatographyparticle sizeEncapsulation efficiencypreparation processelectron microscopy
分类号：R3(基础医学)
资助基金：from the National Natural Science Foundation of China and a grant(30872999)from Jiangsu Province Natural Science Foundation of China(BK2007023)
论文发表日期：2010-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：7( 26-32 )
英文信息