The remedial effect of soluble interleukin-1 receptor type Ⅱ on endometriosis in the nude mouse model
Liying Gao
Liang Sun
Yugui Cui
Zhen Hou
Li Gao
Jing Zhou
Yundong Mao
Suping Han
Jiayin Liu
摘要：Objective: Recent studies have shown that the local expression of soluble interleukin (IL) -1 receptor type Ⅱ (Sil-1 R Ⅱ) in endometrial tissue of women with endometriosis is decreased,and the depression of IL-I RⅡ was more significant in infertile women than that in fertile women with endometriosis. In this research,we investigated the remedial effect of Sil-1-R Ⅱ administration on endometriosis in the nude mouse model. Methods: Nineteen nude model mice with endometriosis were randomly divided into three groups:group A was treated by intraperitoneal administration with only Sil-1 R Ⅱ for two weeks,group B was similarly treated with only IL-1,and group C (control) was administered saline. After 2 weeks,the size of the ectopic endometrial lesions was calculated,and the expression of vascular endothelial growth factor (VEGF) and B-cell lymphoma leukemia-2 (Bcl2) were detected by immunohistocbemistry. The IL-8 and VEGF levels in the peritoneal fluid (PF) and serum were also measured by enzyme-linked immunosorbent assay (ELISA). Results: The mean size of ectopic endometrial lesion did not differ between the three groups (P>0.05). Compared with the control,the expression of VEGF and Bcl-2 was significantly lower in group A,and higher in group B. In the three groups,the levels of IL-8 in the PF and serum were highest in group A,and lowest in group B. Conclusion: sIL-1 RⅡ may suppresse hyperplasia of ectopic endometriosis,perhaps by reducing the expression of certain cytokines,such as VEGF,IL-8,and Bcl-2,which could provide a new clinical strategy for the treatment of endometriosis.
机标关键词：typereceptormouse modelendothelial growth factorVEGFperitoneal fluidwomenthree
分类号：R3(基础医学)
资助基金：funding from Innovative Research Team in Nanjing Medical University(IRT0631)the collaborating Grants(30611120524)
论文发表日期：2010-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：8( 43-50 )
英文信息