Construction and identification of recombinant lentiviral vector containing HIV-1 Tat gene and its expression in 293T cells
Bingbing Wei
Ninghan Feng
Feng Zhou
Chun Lu
Jiantang Su
Lixin Hua
摘要：Objective: To construct a lentiviral vector expressing HIV-1 Tat and identify its expression in 293T cells.Methods: The gene fragment of HIV-I Tat101 was subcloned to lentiviral transfer vector pHAGE-CMV-MCS-IZsGreen,which was named pHAGE-Tat. Then the constructed pHAGE-Tat was used to co-transfect the packing 293T cells,together with the packaging plasmids pMD2.G and psPAX2. The packaged viral particles designated LV-Tat were used to infect the 293T cells and the viral titer was calculated. The expression of HIV-1 Tat in 293T cells was confirmed using RT-PCR and western blot. Results: The recombinant lentiviral vector was successfully constructed and could express HIV-1 Tat in 293T cells. The virus titer was 5.73×106 ifu/ml. Conclusion: The successfully constructed recombinant lentiviral vector makes a strong foundation for further exploring the possible role of HIV-1 Tat in the development of prostate cancer.
机标关键词：cellsHIV-1 Tatlentiviral vectorwestern blot.possible rolevirus titerdevelopmentexploring
分类号：R3(基础医学)
资助基金：China Postdoctoral Foundation funded project(20080441064)
论文发表日期：2010-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
页数：6( 58-63 )
英文信息