Activation of calcium-sensing receptors is associated with apoptosis in a model of simulated cardiomyocytes ischemia/reperfusion
Ling Yan
Tiebing Zhu
Tingting Sun
Liansheng Wang
Shiyang Pan
Zhengxian Tao
Zhijian Yang
Kejiang Cao
摘要：Objective: Calcium-sensing receptors(CaSRs)are G-protein coupled receptors which maintain systemic calcium homeostasis and participate in hormone secretion,activation of ion channels,cell apoptosis,proliferation,and differentiation.Previous studies have shown that CaSRs induce apoptosis in isolated adult rat heart and in normal neonatal rat cardiomyocytes by G-protein-PLC-IP3 signaling transduction.However,little knowledge is presently available concerning the role of CaSRs in the apoptosis induced by ischemia and reperfusion in neonatal cardiomyocytes.Methods: Primary neonatal rat ventricular cardiomyocytes were incubated in ischemiamimetic solution for 2 h,and then re-incubated in normal culture medium for 24 h to establish a model of simulated ischemia/reperfusion(I/R).Cardiomyocyte apoptosis was detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL).The expression of CaSRs mRNA was detected by real-time reverse transcription polymerase chain reaction(RT-PCR).In addition,the expressions of caspase-3 and Bcl-2 were analyzed by western blot.Results: The simulated I/R enhanced the expression of CaSRs and cardiomyocyte apoptosis.GdCl3,a specific activator of CaSRs,further increased the expression of CaSRs and cardiomyocyte apoptosis,along with up-regulation of caspase-3 and down-regulation of Bcl-2.Conclusion: CaSRs are associated with I/R injury and apoptosis in neonatal rat ventricular cardiomyocytes via suppressing Bcl-2 and promoting caspase-3 expression.
机标关键词：cardiomyocyte apoptosisneonatal ratcaspase-3polymerase chain reactionischemia and reperfusionreverse transcriptioncalcium homeostasisBcl-2
分类号：R3(基础医学)
资助基金：the Nature Science Foundation of the Education Department of Jiangsu Province(06kjb320006)
论文发表日期：2010-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
英文信息