Resveratrol prevents interleukin-1β-induced dysfunction of pancreatic β-cells
Fang Chen
Xiaohua Zhou
Yan Lin
Changwen Jing
Tao Yang
Yong Ji
Yujie Sun
Xiao Han
摘要：Objective:Interleukin-1β(IL-1β)plays an important role in the development of type 1 and type 2 diabetes mellitus.Resveratrol,a polyphenol,is known to have a wide range of pharmacological properties in vitro.In this research,we examined the effects of resveratrol on IL-1β-induced β-cell dysfunction.Methods: We first evaluated the effect of resveratrol on nitric oxide(NO)formation in RINm5F cells stimulated with IL-1β using the Griess method.Next,we performed transient transfection and reporter assays to measure the transcriptional activity of peroxisome proliferator-activated receptor-γ(PPAR-γ).We also used Western blotting analysis to assess the effect of resveratrol on inducible nitric oxide synthase(iNOS)expression and nuclear factor-κB(NF-κB)translocation to the nuclei in cells treated with IL-1β.In addition,we assessed the transcriptional activity of NF-κB using an electrophoretic mobility shift assay(EMSA).Finally,we evaluated the effect of resveratrol on IL-1β-induced inhibition of glucose-stimulated insulin secretion in freshly isolated rat pancreatic islets.Results: Resveratrol significantly suppressed IL-1β-induced NO production,a finding that correlated well with reduced levels of iNOS mRNA and protein.The molecular mechanism by which resveratrol inhibited iNOS gene expression appeared to involve increased PPAR-γ activity,which resulted in the inhibition of NF-κB activation.Further analysis showed that resveratrol could prevent IL-1β-induced inhibition of glucose-stimulated insulin secretion in rat islets.Con-clusion: In this study,we demonstrated that resveratrol could protect against pancreatic β-cell dysfunction caused by IL-1β.
机标关键词：IL-1βinsulin secretionnitric oxide synthasemolecular mechanismWestern blottinganalysisgene expressionPPAR-γ
分类号：R3(基础医学)
资助基金：This study was supported by grants from the National Natural Science Foundation of China(30370676;30771041)the Special Funds for Major State Basic Research Program of China(973Program)(2006CB503908)
论文发表日期：2010-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
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