Effects of propofol on early and late cytokines in lipopolysaccharide-induced septic shock in rats

Sha Li; Hongguang Bao; Liu Han; Lele Liu

摘要：Objective:It has been reported that the intravenous anesthetic propofol(PPF)has anti-inflammatory effects in vitro and in patients.The purpose of this study was to investigate whether PPF has anti-inflammatory effects in lipopolysaccharide(LPS)-induced septic shock by inhibiting the induction of pro-inflammatory cytokines[inter-leukin-6(IL-6)and tumor necrosis factor-α(TNF-α)]and high mobility group box 1(HMGB1)in rats.Methods:Thirty six male Wistar rats were randomly assigned to one of three groups(control group,PPF+LPS group and LPS group; n=12 per group).Control group rats received a 0.9% NaCl solution(NS)by the tail vein.The PPF+mg/kg)via the tail vein.Hemodynamic effects were recorded as well as mortality rates,and plasma cytokine concentrations(TNF-α,IL-6,HMGB1)were measured for the 24-h observation period.Results: The mean arterial pressure and heart rate of the PPF+LPS group were more stable than those of the LPS group.The mortality at 24h after the administration of the LPS injection was much higher in the LPS group(58.3%)compared to the PPF+LPS group(25.0%).Plasma concentrations of cytokines(IL-6 and TNF-α)and HMGB1 were significantly reduced in the PPF+LPS group compared to the LPS group(P<0.05).Conclusion: Pretreatment with PPF reduced the mortality rate of rats and attenuated the pro-inflammatory cytokine responses in an endotoxin shock model through an anti-inflammatory action inhibiting induction of HMGB1.