Characterization and potential diagnostic application of monoclonal antibodies specific to rabies virus
Xinjian Liu
Xiaomin Feng
Qi Tang
Zhongcan Wang
Zhenning Qiu
Yuhua Li
Changjun Wang
Zhenqing Feng
Jin Zhu
Xiaohong Guan
摘要：Objective:Rabies is invariably a fatal encephalomyelitis that is considered to be a serious public health problem.It is necessary to develop standard rabies virus diagnostic tools,especially for diagnosing the strains prevalent in China.Methods: Monoclonal antibodies(Mabs)specific to rabies virus were produced and characterized by enzyme linked immunosorbent assay(ELISA),isotyping,affinity assay,immunofluorescence assay(IFA),and immunocytochemistry.The Mab,whose affinity was higher for antigen,was used to establish an antigen capture-ELISA(AC-ELISA)detection system and test the efficiency by using clinical samples.Results: The heavy chain saeclasses of two Mabs were all determined to be IgG2a.The 3C7 Mab showed stronger reactivity with rabies virus protein than the 2C5 Mab in an ELISA analysis,whereas the 3C7 Mab showed the highest affinity for antigen.IFA and immunocytochemistry results also indicated that the two Mabs could recognize rabies virus protein in its native form in cell samples.Data obtained using clinical samples showed that rabies virus could be detected by AC-ELISA detection system using the 3C7 Mab.Conclusion: It was potentially useful for the further development of highly sensitive,easily handled,and relatively rapid detection kits/tools for rabies surveillance in those areas where rabies is endemic,especially in China.
机标关键词：monoclonal antibodiesapplicationrapid detectionlinked immunosorbent assayAC-ELISAespeciallyMabresults
分类号：R3(基础医学)
资助基金：This work was supported by the National High Technology Research and Development Program of China(863 Program)(2007AA02Z418)
论文发表日期：2010-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
英文信息