MiR-181b suppresses proliferation of and reduces chemoresistance to temozolomide in U87 glioma stem cells
Ping Li
Xiaoming Lu
Yingyi Wang
Lihua Sun
Chunfa Qian
Wei Yan
Ning Liu
Yongping You
Zhen Fu
摘要：MicroRNAs regulate self renewal and differentiation of cancer stem cells. There, we sought to identify the expression of miR-181b in glioma stem cells and investigate the biological effect of miR-181b on glioma stem cells in this study. MiR-181b expression was measured by real-time PCR in glioma stem cells isolated from U87 cells by FACS sorting. After miR-181b was overexpressed in U87 glioma stem cells by miR-181b lentiviral expression vector and/or treatment of temozolomide, secondary neurosphere assay, soft agar colony assay and MTT assay were performed. Compared with U87 cells, the expression of miR-181b was significantly decreased in U87 glioma stem cells. Overex1pression of miR-181b decreased neurosphere formation by U87 glioma stem cells in vitro and suppressed colony formation in soft agar, and the cell growth inhibition rates increased in a time-dependent manner in U87 glioma stem cells infected with miR-181b lentivirus. Furthermore, miR-181b had a synergistic effect on temozolomide-induced inhibition of secondary neurosphere and soft agar colony, and on cell growth inhibition rates. MiR-181b functions as a tumor suppressor that suppresses proliferation and reduces chemoresistance to temozolomide in glioma stem cells.
机标关键词：stem cellsgrowth inhibitionsynergistic effectexpression vectorbiological effecttumor suppressorreal-time PCRsoft
分类号：R3(基础医学)
资助基金：the China Natural Science Foundation(30872657)Jiangsu Province's Medical Major Talent Foundation(RC2007061)Jiangsu Province's Natural Science Foundation(2008475)Jiangsu Province's "333" Key Talent Foundation(0508RS08)
论文发表日期：2010-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
英文信息