Human herpesvirus 6A induces apoptosis of HSB-2 cells via a mitochondrion-related caspase pathway
Lingyun Li
Jing Chi
Feng Zhou
Dandan Guo
Fang Wang
Genyan Liu
Chun Zhang
Kun Yao
摘要：Apoptosis plays an important role in the pathogenesis of viral infections. In this study, we investigated the cell death processes during productive HHV-6A infection and the underlying mechanisms. Annexin V-PI staining and electron microscopy indicated that HHV-6A is a strong inducer of apoptosis. HHV-6A infection decreased mitochondrial transmembrane potential and led to morphological changes of mitochondria. The cell death was associated with activation of caspase-3 and cleavage of DNA repair enzyme poly (ADP-ribose) polymerase, which is known to be an important substrate for activated caspase-3. Caspase-9 was activated significantly in HHV-6A-infected cells, whereas caspase-8 was not activated obviously. Moreover, HHV-6A infection upregulated Bax and downregulated Bcl-2. This is the first demonstration of mitochondrion-mediated, caspase-dependent apoptosis in HHV-6A-infected cells.
机标关键词：caspase-3viacellsmorphological changescell deathelectron microscopyDNA repairprocesses
分类号：R3(基础医学)
资助基金：the National Natural Science Foundation of China(30771961;30901344)Science Development Foundation of Nanjing Medical University(08NMUZ003)Jiangsu Province Laboratory of Pathogen Biology(08bykf01)
论文发表日期：2010-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
英文信息