A novel SATB1 binding site in the BCL2 promoter region possesses transcriptional regulatory function
Feiran Gong
Luan Sun
Yujie Sun
摘要：BCL2 is a key regulator of apoptosis. Our previous work has demonstrated that special AT-rich sequencebinding protein 1 (SATB 1) is positively correlated with BCL2 expression. In the present study, we report a new SATB1 binding site located between P1 and P2111 promoters of the BCL2 gene. The candidate SATB1 binding sequence predicted by bioinformatic analysis was investigated in vitro and in vivo by electrophoretic gel mobility shift assays (EMSA) and chromatin immunoprecipitation (ChIP). One 25-bp sequence, named SB1, was confirmed to be SATB1 binding site. The regulatory function of SB1 and its relevance to SATB1 were further examed with dual-luciferase reporter assay system in Jurk1at cells. We found that SB 1 could negatively regulate reporter gene activity. Mutation of SATB1 binding site further repressed the activity. Knockdown of SATB1 also enhanced this negative effect of SB1. Our data indicate that the SB1 sequence possesses negative transcriptional regulatory function and this function can be antagonized by SATB1.
机标关键词：functionregionBCL2binding siteSATB1chromatin immunoprecipitationnegative effectreporter gene
分类号：R3(基础医学)
资助基金：the National Natural Science Foundation of China(30772490)Special Major National Natural Science Foundation of China(90919051)
论文发表日期：2010-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
英文信息