Comparative domain modeling of human EGF-like module EMR2 and study of interaction of the fourth domain of EGF with chondroitin 4-sulphate
Mukta Rani
Manas R. Dikhit
Ganesh C Sahoo
Pradeep Das
摘要：EMR2 is an EGF-like module containing mucin-like hormone receptor-2 precursor, a G-protein coupled recep-tor (G-PCR). Mutation in EMR2 causes complicated disorders like polycystic kidney disease (PKD). The struc-ture of EMR2 shows that the fifth domain is comprised of EGF-TM7 helices. Functional assignment of EMR2 by support vector machine (SVM) revealed that along with transporter activity, several novel functions are predicted. A twenty amino acid sequence "MGGRVFLVFLAFCVWLTLPG" acts as the signal peptide responsible for post-translational transport. Eight amino acids are involved in N-glycosylation sites and two cleavage sites are Leu517 and Ser518 in EMR2. The residue Arg241 is responsible for interaction with glycosaminoglycan and chondroitin sulfate. On the basis of structure, function and ligand binding sites, competitive EMR2 inhibitors designed may decrease the rate of human diseases like Usher's syndrome, bilateral frontoparietal polymicrogyria and PKD.
机标关键词：EGFdomain modelinginteractionstudyhumanpolycystic kidney diseasesupport vector machineamino acid sequence
分类号：R692.1(泌尿科学（泌尿生殖系疾病）)
论文发表日期：2011-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
英文信息