Fibrinogen-like protein 2 expression correlates with microthrombosis in rats with type 2 diabetic nephropathy
Guanhua Su
Kun Liu
Yan Wang
Jue Wang
Xiaowei Li
Wenzhu Li
Yuhua Liao
Zhaohui Wang
摘要：Fibfinogen-like protein 2 (fg12), a novel prothrombinase, is involved in microthrombosis. We examined fg12 expression in the glomerular and tubulointerstitial capillaries and its correlation with microthromsis in rats with streptozocin-induced type 2 diabetic nephropathy. Our RT-PCR and immunoblotting analysis showed that fgl2 mRNA and protein levels were increased in microvascular endothelial cells of the glomeruli and renal interstitia at week 19 and became significantly elevated with the development of diabetic nephropathy (P<0.01). Fg12 was not or only weakly expressed in the renal tissues of normal rats. Furthermore, a direct significant correlation (r=0.543,P<0.01) was found between fg12 expression and microthrombofic capillaries in the renal tissues. Enzyme linked immunosorbent assays (ELISA) additionally showed that circulating TNF-αlevels in rats with type 2 diabetes were significantly elevated and closely correlated with fg12 expression (r=0.871, P<0.01). Our results suggest that fg12 may activate renal microthrombosis, thus contributing to glomerular hypertension and renal ischemia.
机标关键词：diabetic nephropathycorrelationendothelial cellslevelssignificantdevelopmenttypeanalysis
分类号：R587.2(内分泌腺疾病及代谢病)R692.9(泌尿科学（泌尿生殖系疾病）)
资助基金：This study was supported by CGICC Medical Science Research Sup-porting Program(08010022)National Program on Key Basic Research Project of China(2007CB512000;Sub-Project 2007CB512005)
论文发表日期：2011-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
英文信息