UVB suppresses PTEN expression by upregulating miR-141 in HaCaT cells
Wei Li
Wu Di
Lijuan Hua
Bingrong Zhou
Ze Guo
Dan Luo
摘要：MicroRNAs (miRNAs) are 21 to 24 nucleotide, non-coding RNA molecules that post-transcriptionally regulate the expression of target genes. Ultraviolet B (UVB) radiation has been shown to inhibit phosphatase and tensin homolog deleted on chromosome 10 (PTEN) expression in HaCaT cells through an unknown mechanism. In this study, we investigated whether miR-141 can regulate UVB exposure-mediated inhibition of PTEN expression. Real-time RT-PCR, annexin V/fluorescein isothiocyanate staining, Western blotting and anti-miRNA oligonucle- otide transfection were employed in this study. We found that upregulation of miR-141 expression after UVB ir- radiation was inversely correlated with PTEN expression levels in HaCaT cells. Furthermore, miR-141 expression increased apoptosis, while anti-miR-141 partly restored PTEN expression and reversed the pro-apoptosis, effect of UVB. UVB suppresses the expression of PTEN by upregulating miR-141 in HaCaT cells. Therefore, miR-141 is a potential gene therapy target for UVB-induced photodamage.
机标关键词：cellsHaCaTPTENUVBReal-time RT-PCRtensin homologgene therapystudy
分类号：R739.5(皮肤肿瘤)
资助基金：This study was supported by the National Natural Science Foundation of China(30771946;81000700)
论文发表日期：2011-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
英文信息