MiR-148a inhibits angiogenesis by targeting ERBB3
Jing Yu
Qi Li
Qing Xu
Lingzhi Liu
Binghua Jiang
摘要：MicroRNAs (miRNAs) play an important role in carcinogenesis in various solid cancers including breast cancer. Down-regulation of microRNA-148a (miR-148a) has been reported in certain cancer types. However, the biological role of miR-148a and its related targets in breast cancer are unknown yet. In this study, we showed that the level of miR-148a was lower in MCF7 cells than that in MCF10A cells. V-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (ERBB3) is a direct target of miR-148α in human breast cancer cells through direct binding of miR-148a to ERBB3 3'-UTR region. Overexpression of miR-148a in MCF7 cells inhibited ERBB3 expression, blocked the downstream pathway activation including activation of AKT, ERK1/2, and p70S6K1, and decreased HIF-1α expression. Furthermore, forced expression of miR-148α attenuated tumor angiogenesis in vivo. Our results identify ERBB3 as a direct target of miR-148a, and provide direct evidence that miR-l48a inhibits tumor angiogenesis through ERBB3 and its downstream signaling molecules. This information would be helpful for targeting the miR-148alERBB3 pathway for breast cancer prevention and treatment in the future.
机标关键词：breast cancer cellstumor angiogenesisERBB3prevention and treatmentinformationMCF7resultsrelated
分类号：Q782(基因工程（遗传工程）)
资助基金：This work was supported by grants of the National Natural Science Foundation of China(308712;;81071642)
论文发表日期：2011-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
英文信息