TSP-1 promotes glomerular mesangial cell proliferation and extracellular matrix secretion in Thy-1 nephritis rats
Wen Qiu
Yah Li
Jianbo Zhou
Chenhui Zhao
Jing Zhang
Kai Shan
Dan Zhao
Yingwei Wang
摘要：The proliferation of glomerular mesangial cells (GMC) and secretion of the extracellular matrix (ECM) in rat with Thy-1 nephritis (Thy-1N) resembling human mesangioproliferative glomerulonephritis have been explored for many years; however,the molecular mechanisms of GMC proliferation and ECM production remain unclear.Our previous studies have demonstrated that the thrombospondin-1 (TSP-1) gene was involved in mediating rat GMC proliferation and ECM synthesis induced by sublytic C5b-9 in vitro.In the present study,the roles of the TSP-1 gene in GMC proliferation,ECM production,and urinary protein secretion in Thy-1N rats were determined by using TSP-1 small hairpin RNA,and the results revealed that silencing of the TSP-1 gene in rat renal tissues could diminish GMC proliferation (P ＜ 0.01) and ECM secretion (P ＜ 0.01) as well as urinary protein secretion (P ＜ 0.05) in Thy-1N rats.Together,the current findings suggested that TSP-1 gene expression was required for GMC proliferation and ECM production in Thy-1N rats.
机标关键词：Thy-1extracellular matrixcell proliferationglomerular mesangial cellsTSP-1molecular mechanismsECMsmall hairpin RNA
分类号：R692.3(泌尿科学（泌尿生殖系疾病）)
资助基金：National Natural Science Foundations of China(31000396;;81072402)Natural Science Foundations of Jiangsu Province in China(BK2009417;10KJB310006;;09hx43)
论文发表日期：2011-01-01
在线出版日期：2025-08-15（本平台首次上网日期，不代表文献的发表时间）
英文信息